![]() Statistical analysis using correlation coefficients, Passing-Bablok regression analysis, and Bland-Altman plots found good agreement between results from samples run immediately after phlebotomy and 4 hr postsampling on the i-STAT PCA with the exception of K⁺, which is known to change with sample storage. Analytes evaluated were hydrogen ion concentration (pH), glucose, potassium (K⁺), sodium (Na⁺), bicarbonate (HCO₃⁻), total carbon dioxide (TCO₂), partial pressure of carbon dioxide (PCO₂), and ionized calcium (iCa²⁺). In addition, the effect of sample storage was evaluated on the i-STAT PCA. In this study, the i-STAT PCA was compared with the Radiometer ABL 800 Flex benchtop analyzer using 24 heparinized whole blood samples obtained from healthy E. This analyzer is commonly used in nondomestic animals however, validation of this method in comparison with traditional benchtop methods should be performed for each species. Thei-STAT® portable clinical analyzer (PCA) provides patient-side results for hematologic, biochemical, and blood gas values when immediate results are desired. This study supports the use of the i-STAT PCA for the evaluation of these analytes, with the exception of K⁺, in the Asian elephant. Within the reference range for iCa²⁺, the iCa²⁺ values obtained by the i-STAT PCA and Radiometer ABL 800 Flex were close in value, however in light of the constant and proportionate biases detected, overestimation at higher values and underestimation at lower values of iCa²⁺ by the i-STAT PCA would be of potential concern. Despite bias, mean differences assessed via Bland-Altman plots were clinically acceptable for all analytes excluding K⁺. Comparison of the results from the two analyzers at 4 hr postsampling found very strong or strong correlation in all values except K⁺, with statistically significant bias in all values except glucose and PCO₂. ![]() In particular, the i-STAT predicted hypocalcemia with a high sensitivity.Thei-STAT® portable clinical analyzer (PCA) provides patient-side results for hematologic, biochemical, and blood gas values when immediate results are desired. ICa 2+ concentrations in bovine blood measured with the i-STAT agreed with those of the Radiometer ABL800 FLEX. The Bland-Altman difference plot revealed no bias between the i-STAT and the Radiometer ABL 800 FLEX. The deviation from the identity line was not significant ( P = 0.64). The Passing-Bablok regression equation that fit the iCa 2+ concentration as measured with the i-STAT ( Y) and the Radiometer ABL800 FLEX ( X), was Y = −0.108276 + 1.103448 × X, with a residual deviation of 0.02. The i-STAT performance was evaluated at two different cutoff values for iCa 2+ concentrations (<1.00 and <1.18 mmol/L) using the receiver operating characteristic curve. The Radiometer ABL800 FLEX was the reference method and the i-STAT was the test method. Data were subjected to Passing-Bablok regression and Bland-Altman plots. Venous blood samples were run on the i-STAT and the Radiometer ABL800 FLEX for determination of blood iCa 2+ concentrations. In this study, 121 cattle with various diseases were used. The primary goal of this study was to evaluate the agreement of the i-STAT against a traditional bench-top blood gas analyzer (Radiometer ABL800 FLEX) in the measurement of iCa 2+ in the blood of cattle with various disorders. Information regarding the performance of the i-STAT, a point-of-care analyzer, to determine ionized calcium (iCa 2+) concentration in bovine blood is limited.
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